The goal of this activity was to determine how to differentiate the types of saccharides based on how they reacted in iodine and Benedict's solutions.
Procedure:
Mono, Di, and Poly saccharides were tested for reactions in iodine and Benidict's solutions. Several sugar samples were then tested for reactions in the same solutions. The type of saccharide was determined based on the samples' reactions when mixed with the two solutions compared to the reactions of the pure saccharides when mixed with the solutions. If there was a reaction between the saccharide and the iodine, the reaction occurred immediately. The Benedict's solution needed to be heated with the saccharide before a reaction could occur.
Claims:
Based off of the evidence produced during the procedure, it can be determined that if a saccharide reacts to the Benidict's solution and not the iodine solution, it can be positively identified as a monosaccharide. If a saccharide reacts to the iodine solution and not the Benedict's solution, it can be positively identified as a polysaccharide. If a saccharide does not react to either solution, it can be positively identified as a disaccharide. Each type of saccharide could be identified based off of their reactions in the two solutions.
Evidence:
The claims of this experiment can be proven with the evidence gained throughout the procedure. The tables below display the evidence gathered from the experiment. (+) indicates a reaction while (-) indicates no visible change.
Sample Benedict's Iodine
Monosaccharide + -
Disaccharide - -
Polysaccaride - +
Sample Benedict's Iodine
Powerade + -
Corn Starch - +
Niagara Spray Starch - +
Kix Cereal - +
Galactose + -
Sample Benedict's Iodine
Monosaccharide + -
Disaccharide - -
Polysaccaride - +
Sample Benedict's Iodine
Powerade + -
Corn Starch - +
Niagara Spray Starch - +
Kix Cereal - +
Galactose + -
Research:
When compared to other groups' findings, nutrition facts, information gathered in class, and web sources, it is clear that similar conclusions were, or could be drawn from this experiment.
Reflection:
Through this experiment, ideas have remained consistent throughout the discovery of new findings. Though new questions were rasied throughout the scientific process, each could be traced back to the original hypothesis that the saccharides could be successfully differentiated based on the chemical reactions visualized when mixed with each solution. Improvement in the experiment could include the testing of known poly, di, and mono saccharides to prove their saccharide content.
Lab Analysis Questions:
Further explanations on this lab topic can be found at this address:
Pictures from the Experiment:
These pictures were taken directly from the experiment without other sources.
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